Journal: eLife
Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation
doi: 10.7554/eLife.38883
Figure Lengend Snippet: ( A ) Coomassie Brilliant Blue (CBB) staining and western blotting of purified EGFL6, RGE-mutant EGFL6, and nephronectin (NPNT) are shown. ( B ) Heat map display of the results of cell adhesion assays. The number of adhered cells was converted to arbitrary units (A.U.). FN, fibronectin; LN511-E8, laminin-511 E8 fragment. n = 3. Cell lines used are human primary keratinocytes, human embryonic kidney epithelial cell line HEK293, human glioblastoma cell line T98G, Human glioma cell line KG-1-C, human skin fibroblast cell line SF-TY, human embryonic fibroblast cell line NTI-4, human sarcoma cell line HT-1080, human myelogenous leukemia cell line K562 and K562 transfected with human integrin α8 cDNA K562-a8. ( C ) Cell adhesion assays with T98G and KG-1-C cells plated on EGFL6 or RGE-mutant EGFL6-coated dishes. Data are mean ± SEM, n = 3. ( D ) A maximum projection image of 8-week-old telogen dorsal hair follicles stained for EGFL6, integrin α8 and βIII-tubulin. Scale bar, 50 μm. Integrin α8 was detected in arrector pili muscles (white arrow), periphery of dermal papilla (open arrowheads) and periphery of lanceolate complexes (see the inset), but not in EGFL6-positive lanceolate complexes (see the inset). ( E ) Cell adhesion inhibition assays with integrin antibodies. Data are mean ± SEM, n = 3. ( F ) 8-week-old telogen dorsal hair follicles of wild-type and Egfl6 knockout mice were stained for EGFL6, integrin αv and nestin. Scale bar, 10 μm. Blue dashed lines indicate the surface of follicle epidermis and white dashed lines indicate cross-section positions. ( G ) Quantification of αv integrin signal intensity in lanceolate complexes. Data are mean ± SD. ( H ) 8-week-old telogen dorsal hair follicles of wild-type mice were stained for nestin, βIII-tubulin, integrins αv, β1, α3 and α6. Sagittal view ( x–y ) and transverse view ( x–z ) images are shown. HF, hair follicle. Scale bar, 5 μm. Statistics ( C, E, G ): two-tailed unpaired t -test. 10.7554/eLife.38883.011 Figure 3—source data 1. Raw numerical data for .
Article Snippet: Cell lines used in this study were 293F cells (Thermo Fisher), human primary keratinocytes (Watt lab), human embryonic kidney epithelial cell line HEK293 (RIKEN Bioresource Center), human glioblastoma cell line T98G (JCRB Cell Bank), Human glioma cell line KG-1-C (JCRB Cell Bank), human skin fibroblast cell line SF-TY (JCRB Cell Bank), human embryonic fibroblast cell line NTI-4 (JCRB Cell Bank), human sarcoma cell line HT-1080 (JCRB Cell Bank), human myelogenous leukemia cell line K562 (JCRB Cell Bank) and human myelogenous leukemia cell line transfected with human integrin α8 cDNA K562-a8 (Gift from L. Reichardt).
Techniques: Staining, Western Blot, Purification, Mutagenesis, Transfection, Muscles, Inhibition, Knock-Out, Two Tailed Test
